연구단계 | 1단계 :1년차 | ||
논문제목(영문) | Restoration of angiogenic capacity of diabetes-insulted mesenchymal stem cells by oxytocin. | ||
국내외구분 | 국외 | SCI여부 | SCI |
연구책임자역활 | 교신저자 | 논문기여율 | 30% |
주저자명 | Kim YS | ||
교신저자명 | Ahn Y | ||
공동저자명 | Kwon JS, Hong MH, Kang WS, Jeong HY, Kang HJ, Jeong Mh | ||
게제년월일 | 2013-09-11 | ||
ISSN | 1471-2121 | ||
Impact Factor | 2.844 | ||
학술지명 | BMC CELL BIOL | ||
서지사항 | 0집 / 14권 / 0호, 페이지(38 - 38) | ||
병기표기 | 단독 | ||
Acknowledgement 기재여부 |
예
※ Acknowledgement가 기재된 논문만 연구과제의 성과로 인정. - 국문 표기 : "본 연구는 보건복지부 보건의료연구개발사업의 지원에 의하여 이루어진 것임. (HI13C1527)" - 영문 표기 : "This study was supported by a grant of the Korean Health Technology R&D Project, (HI13C1527) Ministry of Health & Welfare, Republic of Korea. " |
||
요약초록문 (Abstract) 입력 |
(Abstract) 입력 BACKGROUND: Angiogenesis is the main therapeutic mechanism of cell therapy for cardiovascular diseases, but diabetes is reported to reduce the function and number of progenitor cells. Therefore, we studied the effect of streptozotocin-induced diabetes on the bone marrow-mesenchymal stem cell (MSC) function, and examined whether diabetes-impaired MSC could be rescued by pretreatment with oxytocin. RESULTS: MSCs were isolated and cultured from diabetic (DM) or non-diabetic (non-DM) rat, and proliferation rate was compared. DM-MSC was pretreated with oxytocin and compared with non-DM-MSC. Angiogenic capacity was estimated by tube formation and Matrigel plug assay, and therapeutic efficacy was studied in rat myocardial infarction (MI) model.The proliferation and angiogenic activity of DM-MSC were severely impaired but significantly improved by pretreatment with oxytocin. Krüppel-like factor 2 (KLF2), a critical angiogenic factor, was dramatically reduced in DM-MSC and significantly restored by oxytocin. In the Matrigel plug assay, vessel formation of DM-BMSCs was attenuated but was recovered by oxytocin. In rat MI model, DM-MSC injection did not ameliorate cardiac injury, whereas oxytocin-pretreated DM-MSC improved cardiac function and reduced fibrosis. CONCLUSIONS: Our results show that diabetes influenced MSC by reducing angiogenic capacity and therapeutic potential. We demonstrate the striking effect of oxytocin on stem cell dysfunction and suggest the use of oxytocin as a priming reagent in autologous stem cell therapy. |